在食藥用菇類菌絲體線性生長速率上，ODA對香菇台農一號、雲芝、杏鮑菇、金針菇、巴西洋菇菌絲有促進生長的作用。在雲芝、杏鮑菇及金針菇中促進生長效果分別為添加10 ppm >5 ppm>2.5 ppm>控制組，且10 ppm之ODA添加量可使雲芝、杏鮑菇、金針菇之相對生長速率分別為1.472、1.890及1.251。ODA對香菇台農一號和巴西洋菇菌絲有促進生長作用，但在不同處理組之間則不顯著；但ODA對香菇271及草菇的作用在處理組與控制組沒有差異。
在杏鮑菇菌絲體發酵培養上，培養至第五天時殘糖下降量趨於平緩，添加ODA可使菌絲提高對培養基中的糖吸收因而提高菌絲乾重。在添加10 ppm ODA中可提高菌絲產量138%。
Edible and medicinal mushrooms have long been used as food. Because they are rich in dietary fiber, protein, vitamins, chitin, and minerals, they are popular health food. The fruit bodies and mycelia of edible and medicinal mushrooms can be used as foods, condiments, special chemicals, such as soluble polysaccharides, vitamins, organic acids, nucleotides, enzymes etc. and medical materials such as antitumor and antidisease drugs. Therefore, they have gradually become highly valuable produce, which are developed by countries all over the world.
10-Oxo-trans-8-decenoic acid (ODA) is a product formed concurrently with 1-octen-3-ol from linoleic acid by the action of lipoxygenase and hydroperoxide lyase when the mushroom tissue is damaged or disrupted. 1-Octen-3-ol is the principal characteristic of mushroom flavor, and ODA that is a biologically active substance or growth hormone of mushrooms, promotes mycelial growth and stipe elongation. The objectives of the present research were to study the effects of ODA on the growth of edible and medicinal mushroom mycelia on PDY agar, in liquid culture, and in jar-fermentor.
On the linear growth rate of edible and medicinal mycelium, ODA had significant effects on the mycelial growth of Agaricus blazei, Coriolus versicolor, Flammulina velutipes, Lentinula edodes (Tainung 1), and Pleurotus eryngii. The effects of ODA on C. versicolor, F. velutipes, and P. eryngii were in order of 10 ppm> 5 ppm> 2.5 ppm ODA> control. Among the treatments, 10 ppm ODA could increase relative linear growth rate of C. versicolor, P. eryngii, and F. velutipes to 1.472, 1.890, and 1.251, respectively. ODA could promote mycelia growth of A. blazei and L. edodes (Tainung 1), but no significant difference was observed among treatments. However, the effects of ODA on L. edodes (271), and Volvariella volvacea were not significantly different among treatments.
In liquid culture of edible and medicinal mycelium, it was found that the higher the concentrations of ODA, the higher the dry biomasses and the laccase activity of C. versicolor, P. eryngii, and F. velutipes. At 10 ppm ODA, the dry biomasses were about 121%, 123%, and 114%, and the laccase activity increased 156%, 178%, and 153% for C. versicolor, P. eryngii, and F. velutipes mycelia growth compared with the control, respectively.
In jar-fermentor of P. eryngii, it was found that the rate of decrease in the residual sugar slowed down till 5th day. Because adding ODA increased mycelia assimilation of sugar in medium, the dry biomass also increased. At 10 ppm ODA, the biomass was about 138% compared with the control.
It can reduce the culturing time and increase production by applying ODA for bottle cultivation of mushroom such as A. blazei, C. versicolor, F. velutipes, L. edodes (Tainung 1), and P. eryngii and fermentation industry such as P. eryngii. It is more economical and beneficial for the development on food industry.