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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 農藝學系 > 依資料類型分類 > 期刊論文 >  Comparisons of alpha-amylase inhibitors from seeds of common bean mutants extracted through three phase partitioning

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/125975

標題: Comparisons of alpha-amylase inhibitors from seeds of common bean mutants extracted through three phase partitioning
作者: H.H.Wang;C.L.Chen;T.L.Jeng;J.M.Sung
Contributors: 國立中興大學農藝學系
National Chung Hsing University,Department of Agronomy
Miao-zhen Luo
關鍵字: alpha-Amylase inhibitor;Common bean;Mutants;Three-phase partitioning;TPP;phaseolus-vulgaris;purification;separation;proteins
日期: 2011-10
Issue Date: 2012-10-19 14:25:30 (UTC+8)
摘要: This study compared the inhibitory activity of alpha-amylase inhibitor (alpha AI) extracted from common bean (Phaseolus vulgaris L) variety Hwachia, its nine mutants and two introduced varieties by using three-phase partitioning (TPP). A commercially prepared Phase 2 was also used to serve as a comparative reference. The optimal purification parameters for TPP were 30% saturation ammonium sulphate and pH 5.25. Considerable variations were detected in alpha AI content, total inhibitory activity and specific inhibitory activity of alpha AI purified from different common beans. Mutant SA-05 had the alpha AI inhibitory activity of 6267 units g(-1) dry seed weight, which was higher than Hwachia (5062 units g(-1) dry seed weight) and Phase 2 (3200 units g(-1) dry weight). Moreover, it had an extremely lower IC(50) (0.40 mu g) than Phase 2 (10.22 mu g). Thus, the mutant SA-05 may be used as raw material in commercial preparation of alpha AI extracts for controlling appetite and energy intake. (C) 2011 Elsevier Ltd. All rights reserved.
Relation: Food Chemistry, Volume 128, Issue 4, Page(s) 1066-1071.
Appears in Collections:[依資料類型分類] 期刊論文

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