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National Chung Hsing University Institutional Repository - NCHUIR > 獸醫學院 > 微生物暨公共衛生學研究所 > 依資料類型分類 > 期刊論文 >  Characterization of the monoclonal antibody against classical swine fever virus glycoprotein E(rns) and its application to an indirect sandwich ELISA

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/126061

標題: Characterization of the monoclonal antibody against classical swine fever virus glycoprotein E(rns) and its application to an indirect sandwich ELISA
作者: Wu, Ching-Wei;Chien, Maw-Sheng;Liu, Ting-Yu;Lin, Guang-Jan;Lee, Wei-Cheng;Huang, Chienjin
Contributors: 國立中興大學微生物暨公共衛生學研究所
National Chung Hsing University,Graduate Institute of Microbiology and Public Health
Miao-zhen Luo
關鍵字: Classical swine fever virus;RNase activity;Monoclonal antibody;Sandwich ELISA;rnase activity;pestivirus;vaccine;identification;ribonuclease;expression;immunity
日期: 2011-11
Issue Date: 2012-10-19 15:01:18 (UTC+8)
摘要: Classical swine fever virus (CSFV) E(rns) is an envelope glycoprotein possessing RNase activity. The E(rns)-based enzyme-linked immunosorbent assay (ELISA) has been considered a discriminating diagnostic test for differentiating infected from vaccinated animals. The purpose of this study was to produce a specific monoclonal antibody (MAb) to E(rns) for further developing an indirect sandwich ELISA. The MAb CW813 was shown to specifically recognize both the monomer and dimer forms of Pichia pastoris yeast-expressed E(rns) (yE(rns)). The antigenic site recognized by MAb CW813 was mapped to the region of amino acid residues 101-160 of E(rns) where it was neither a neutralizing epitope nor essential to RNase activity. Furthermore, MAb CW813 was utilized as a capture antibody to develop a yE(rns)-based indirect sandwich ELISA for detecting swine antibody to E(rns). The assay demonstrated a high sensitivity and specificity that may provide an alternative method for developing a diagnostic kit with easy manipulation and low cost.
Relation: Applied Microbiology and Biotechnology, Volume 92, Issue 4, Page(s) 815-821.
Appears in Collections:[依資料類型分類] 期刊論文

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