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National Chung Hsing University Institutional Repository - NCHUIR > 學術研究中心 > 生物科技發展中心 > 依資料類型分類 > 期刊論文 >  Optimization of Enzymatic Synthesis of Cetyl 2-Ethylhexanoate by Novozym (R) 435

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/126090

標題: Optimization of Enzymatic Synthesis of Cetyl 2-Ethylhexanoate by Novozym (R) 435
作者: Chen, Hsiao Ching;Kuo, Chia-Hung;Chen, Hsin Hung;Liu, Yung-Chuan;Shieh, Chwen Jen
Contributors: 國立中興大學生物科技發展中心
National Chung Hsing University,Biotechnology Center
Miao-zhen Luo
關鍵字: Biocatalysis;Cetyl 2-ethylhexanoate;Lipase;Optimization;Response;surface methodology;Wax esters;lipase-catalyzed synthesis;surface methodology rsm;wax ester;water-content;alcoholysis;triolein;media;acid
日期: 2011-12
Issue Date: 2012-10-19 15:15:21 (UTC+8)
摘要: Waxes are esters obtained from long-chain fatty acids and long-chain alcohols which are biodegradable, biocompatible and nontoxic. Seafowl feather oil is a natural wax ester that exists on seafowl feathers. Cetyl 2-ethylhexanoate is the major ingredient of seafowl feather oil. Cetyl 2-ethylhexanoate is widely used in cosmetics as a base oil because of its lubricity, moisture retention and non-toxic properties. An optimal production of cetyl 2-ethylhexanoate by direct esterification of cetyl alcohol with 2-ethylhexanoic acid was developed using an immobilized lipase (Novozym(A (R)) 435) as a catalyst in n-hexane. Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) were employed to evaluate the effects of reaction time, reaction temperature, substrate molar ratio, and enzyme amount on the yield of cetyl 2-ethylhexanoate. The results show that reaction time, reaction temperature, substrate molar ratio, and enzyme amount have significant effects on the yield of the esterification reaction. On the basis of ridge-max analysis, the optimum conditions were as follows: a reaction time of 2.65 days, a reaction temperature of 56.18 A degrees C, a substrate molar ratio of 2.55:1, and an enzyme amount of 251.39%. The predicted and experimental values of molar conversion were 91.95 and 89.75 +/- A 1.06%, respectively.
Relation: Journal of the American Oil Chemists Society, Volume 88, Issue 12, Page(s) 1917-1923.
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