English  |  正體中文  |  简体中文  |  Items with full text/Total items : 43312/67235
Visitors : 2108400      Online Users : 12
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU/NCHU Library IR team.
National Chung Hsing University Institutional Repository - NCHUIR > 學術研究中心 > 生物科技發展中心 > 依資料類型分類 > 期刊論文 >  Display female-specific doublesex RNA interference in early generations of transformed oriental fruit fly, Bactrocera dorsalis (Hendel)

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/126091

標題: Display female-specific doublesex RNA interference in early generations of transformed oriental fruit fly, Bactrocera dorsalis (Hendel)
作者: Chen, Shiu-Ling;Lu, Kuang-Hui;Dai, Shu-Mei;Li, Chia-Hsin;Shieh, Chwen-Jen;Chang, Cheng
Contributors: 國立中興大學生物科技發展中心
National Chung Hsing University,Biotechnology Center
Miao-zhen Luo
關鍵字: doublesex gene;Bactrocera dorsalis;RNA interference;short hairpin;RNA;reverse transcription real-time PCR;sex-determining gene;drosophila-melanogaster;dipteran insects;mammalian-cells;musca-domestica;polymerase-iii;evolution;sirna;tephritidae;expression
日期: 2011-4
Issue Date: 2012-10-19 15:15:23 (UTC+8)
摘要: BACKGROUND: The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most destructive pests in many Asian countries. An effective strategy to reduce fly density in the field is urgently required. Recently, the doublesex of B. dorsalis (Bddsx(f)) has been cloned, and RNA interference (RNAi) indicates that it can reduce the offspring in vitro. In this study, a piggyBac-based construct that generates short hairpin RNA (shRNA) against the female-specific region of Bddsx was introduced into the pest to test the RNAi effects on reproductive functions in vivo. RESULTS: After embryonic injection and backcross, 21 transgenic lines with germline transformation were identified. Genomic DNA analysis showed that the exogenous transgene including short hairpin Bddsx(f) and a DsRed marker had integrated into the genomes of 11 transformed lines. Northern blot analysis indicated the presence of Bddsx(f) short interfering RNA (siRNA) under the control of a U6 promoter in transformed flies. As expected, the specific effects of RNAi led to the delay of egg maturation, and the offspring was significantly reduced. Reverse transcription real-time PCR further demonstrated that in vivo interference not only specifically inhibited the Bddsxf transcript but also repressed expression of the downstream yolk protein gene (Bdyp1). CONCLUSION: The results clearly indicate that RNAi is heritable through the expression of specific siRNA in early generations of transformed oriental fruit fly. These results can broaden the understanding of sex-related developmental mechanisms in the fly, and also offer a possible molecular approach for pest control in the future. (C) 2011 Society of Chemical Industry
Relation: Pest Management Science, Volume 67, Issue 4, Page(s) 466-473.
Appears in Collections:[依資料類型分類] 期刊論文

loading Web of Knowledge data....

Files in This Item:

There are no files associated with this item.



 


學術資源

著作權聲明

本網站為收錄中興大學學術著作及學術產出,已積極向著作權人取得全文授權,並盡力防止侵害著作權人之權益。如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員,將盡速為您處理。

本網站之數位內容為國立中興大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用。

聯絡網站維護人員:wyhuang@nchu.edu.tw,04-22840290 # 412。

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU/NCHU Library IR team Copyright ©   - Feedback