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National Chung Hsing University Institutional Repository - NCHUIR > 生命科學院 > 分子生物學研究所 > 依資料類型分類 > 期刊論文 >  Analysis of sequence and haemagglutinin activity of the HN glycoprotein of Newcastle disease virus

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/128765

標題: Analysis of sequence and haemagglutinin activity of the HN glycoprotein of Newcastle disease virus
作者: Ke, G.M.;Chuang, K.P.;Chang, C.D.;Lin, M.Y.;Liu, H.J.
劉宏仁
關鍵字: neuraminidase glycoprotein;monoclonal-antibodies;nucleotide-sequence;structural features;antigenic sites;protein;fusion;gene;mature;pcr
日期: 2010
Issue Date: 2012-12-07 15:10:36 (UTC+8)
關連: Avian Pathology, Volume 39, Issue 3, Page(s) 235-244.
摘要: Reverse transcription-polymerase chain reaction (RT-PCR) was used to generate sequence data for recent Taiwanese strains of Newcastle disease virus (NDV) isolated from 1999 to 2003, covering the full length of the haemagglutinin-neuraminidase (HN) gene and protein. Nucleotide sequence analysis of the HN gene of these recent isolates revealed that the whole HN gene carries an open reading frame encoding 571 amino acids and possesses a shorter C-terminal extension. Six amino acid substitutions in epitopes on the HN glycoprotein of the recent Taiwanese NDV isolates were also found. All the recent Taiwanese NDV isolates have the amino acid sequence 112RRQKRF117 for the F protein. A phylogenetic tree analysis based on the nucleotide sequences of the F gene revealed that all recent Taiwanese isolates were related to genotype VII viruses. Since the recent Taiwanese NDV isolates exhibited a low level of haemagglutination (HA) activity, we generated two sets of mutants to elucidate whether mutations in the heptad repeat region of the HN protein could affect the HA activity. To demonstrate the presence of the viruses used in the HA test, a real-time RT-PCR was established to determine the copy number of NDV isolates. From sequence analysis, site-directed mutagenesis, and haemadsorption assays, it was found that the HN glycoprotein of recent Taiwanese NDV isolates carrying a substitution at the amino acid residue 81 (I to M) in the heptad repeat region in the stalk domain showed a dramatic decrease in the activity of HA. We infer from these results that a specific amino acid sequence within the heptad repeat region of the stalk is important for the HA activity of the HN glycoprotein.
Relation: Avian Pathology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 劉宏仁

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