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National Chung Hsing University Institutional Repository - NCHUIR > 工學院 > 化學工程學系所 > 依資料類型分類 > 期刊論文 >  Adsorption separation for the extracts from Ginkgo biloba leaves using intermediate polarity resins

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/129017

標題: Adsorption separation for the extracts from Ginkgo biloba leaves using intermediate polarity resins
作者: Lai, S.M.;Chen, R.L.;Suen, S.Y.
關鍵字: Ginkgo biloba L. extracts;flavonoid glycosides;terpene lactones;adsorption separation;intermediate polar resins;column performance;performance liquid-chromatography
日期: 2003
Issue Date: 2012-12-07 15:18:15 (UTC+8)
關連: Journal of Liquid Chromatography & Related Technologies, Volume 26, Issue 17, Page(s) 2941-2960.
摘要: An adsorption separation method using intermediate polar resins (Amberlite XAD-7HP) was applied for concentrating the active ingredients from Ginkgo biloba leaves. First, the crude G. biloba L. extracts were prepared using a Soxhlet extractor operated under an optimal extraction condition (using 160 mL of 70% ethanol aqueous solution for 3 h per 10 g dry leaves). The extraction yield was 25-30% and the purity was 2.5-3.0 wt.% for flavonoid glycosides and 0.5-1.0 wt.% for terpene lactones. Before the column operation, the extracts were dissolved in 10% ethanol and used as the feed solution, which led to a 1.5-2.0 fold concentration and a 10-20% loss for both active ingredients. Then, the column was operated under four steps, i.e., adsorption, washing, elution, and regeneration, in sequence. The results show that using the eluent with a high ethanol concentration ( greater than or equal to 70%) in the isocratic elution mode gave good column performance. Both flavonoid glycosides and terpene lactones were nearly completely eluted, their purities all met the standardized contents of G. biloba L. extracts (24 wt.% for flavonoid glycosides and 6 wt.% for terpene lactones), and their yields were nearly 100%. Finally, for a simulated two-step elution mode (first step: 30% and second step: 70% ethanol), the product purity can be further improved, but at the expense of the product yield.
Relation: Journal of Liquid Chromatography & Related Technologies
Appears in Collections:[依教師分類] 孫幸宜
[依資料類型分類] 期刊論文

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