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National Chung Hsing University Institutional Repository - NCHUIR > 生命科學院 > 生命科學院 > 依資料類型分類 > 期刊論文 >  Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/130742

標題: Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells
作者: Chen, J.T.;Lin, T.S.;Chow, K.C.;Huang, H.H.;Chiou, S.H.;Chiang, S.F.;Chen, H.C.;Chuang, T.L.;Lin, T.Y.;Chen, C.Y.
關鍵字: immunohistochemistry;in situ hybridization;microarray;suppression;subtractive hybridization;type II pneumocyte;epithelial-cells;met protooncogene;primary culture;in-vivo;expression;gene;receptors;identification;pathway;injury
日期: 2006
Issue Date: 2012-12-07 16:06:41 (UTC+8)
關連: American Journal of Respiratory Cell and Molecular Biology, Volume 34, Issue 3, Page(s) 264-273.
摘要: We examined gene expression of hepatocyte growth factor (HGF) and HGF receptor (HGFR), or product of proto-oncogene c-met (c-met), in smokers and nonsmokers with adenocarcinoma (ADC) by suppression subtractive hybridization and microarray techniques. Expression of HGF and c-met was confirmed by RT-PCR. HGF content in the respective tumor mass and nontumor lung tissue was measured by ELISA. HGF in pathologic samples was localized by immunohistochemistry and in situ hybridization. Our results indicate that overexpression of HGFR was frequently detected in ADC cells, whereas overexpression of HGF was detected in alveolar type II (ATII) cells. Overexpression of HGF was correlated with cigarette smoking and tumor stages. In vitro, HGF expression was evaluated in isolated murine ATII cells and in 12 ADC cell lines, and we found that nicotine activated HGF expression in ATII cells and lung cancer cells.
Relation: American Journal of Respiratory Cell and Molecular Biology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 邱繡河
[依教師分類] 陳鴻震
[依教師分類] 周寬基
[依教師分類] 陳鴻震

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