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National Chung Hsing University Institutional Repository - NCHUIR > 生命科學院 > 生物化學研究所 > 依資料類型分類 > 期刊論文 >  Characterization of differences between two Trichogramma wasps by molecular markers

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/130786

標題: Characterization of differences between two Trichogramma wasps by molecular markers
作者: Chang, S.C.;Hu, N.T.;Hsin, C.Y.;Sun, C.N.
胡念台
關鍵字: Trichogramma ostriniae;Trichogramma chilonis;PCR of internal noncoding;transcribed spacer region of rDNA;species-specific PCR;species hymenoptera;biological-control;dna
日期: 2001
Issue Date: 2012-12-07 16:07:35 (UTC+8)
關連: Biological Control, Volume 21, Issue 1, Page(s) 75-78.
摘要: Of the two egg parasitic wasps of the Asian corn borer (Ostrinia furnacalis Guenee) (Lepidoptera: Noctuidae) present in Taiwan, Trichogramma ostriniae Pang & Chen (Hymenoptera: Trichogrammatidae) is the dominant species in corn fields and shows higher parasitism than Trichogramma chilonis Ishii in laboratory studies. Molecular techniques were applied to characterize these two wasps, which are small in size and lack clear morphological differences. Distinct differences in the products of random amplified polymorphic DNA were observed in these two species. The internal noncoding transcribed spacer (ITS) region and the partial products of this region of ribosomal DNA, amplified by polymerase chain reaction (PCR), from these two species varied in length. The sequences of the ITS1 regions of the two wasps shared 86.1% identity. PCR with species-specific primers based on these sequences gave partial ITS1 products of 458 and 322 bp and thus unequivocally differentiated T. ostriniae from T. chilonis. (C) 2001 academic Press.
Relation: Biological Control
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 胡念台

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