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National Chung Hsing University Institutional Repository - NCHUIR > 生命科學院 > 生物化學研究所 > 依資料類型分類 > 期刊論文 >  Activation of the Promoter of the Fengycin Synthetase Operon by the UP Element

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/130808

標題: Activation of the Promoter of the Fengycin Synthetase Operon by the UP Element
作者: Ke, W.J.;Chang, B.Y.;Lin, T.P.;Liu, S.T.
張邦彥
關鍵字: polymerase alpha-subunit;multifunctional peptide synthetases;rrnb p1;promoter;bacillus-subtilis;rna-polymerase;escherichia-coli;transcription initiation;nonribosomal synthesis;tyrocidine synthetase;bacterial promoters
日期: 2009
Issue Date: 2012-12-07 16:08:07 (UTC+8)
關連: Journal of Bacteriology, Volume 191, Issue 14, Page(s) 4615-4623.
摘要: Bacillus subtilis F29-3 produces an antifungal peptidic antibiotic that is synthesized nonribosomally by fengycin synthetases. Our previous work established that the promoter of the fengycin synthetase operon is located 86 nucleotides upstream of the translational initiation codon of fenC. This investigation involved transcriptional fusions with a DNA fragment that contains the region between positions -105 and +80 and determined that deleting the region between positions -55 and -42 reduces the promoter activity by 64.5%. Transcriptional fusions in the B. subtilis DB2 chromosome also indicated that mutating the sequence markedly reduces the promoter activity. An in vitro transcription analysis confirmed that the transcription is inefficient when the sequence in this region is mutated. Electrophoretic mobility shift and footprinting analyses demonstrated that the C-terminal domain of the RNA polymerase alpha subunit binds to the region between positions -55 and -39. These results indicated that the sequence is an UP element. Finally, this UP element is critical for the production of fengycin, since mutating the UP sequence in the chromosome of B. subtilis F29-3 reduces the transcription of the fen operon by 85% and prevents the cells from producing enough fengycin to suppress the germination of Paecilomyces variotii spores on agar plates.
Relation: Journal of Bacteriology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 張邦彥

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