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National Chung Hsing University Institutional Repository - NCHUIR > 生命科學院 > 生物化學研究所 > 依資料類型分類 > 期刊論文 >  The 1.5 angstrom structure of endo-1,3-beta-glucanase from Streptomyces sioyaensis: evolution of the active-site structure for 1,3-beta-glucan-binding specificity and hydrolysis

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/130864

標題: The 1.5 angstrom structure of endo-1,3-beta-glucanase from Streptomyces sioyaensis: evolution of the active-site structure for 1,3-beta-glucan-binding specificity and hydrolysis
作者: Hong, T.Y.;Hsiao, Y.Y.;Meng, M.;Li, T.T.
孟孟孝;李天雄
關鍵字: licheniformis 1,3-1,4-beta-d-glucan 4-glucanohydrolase;crystal-structure;biochemical-characterization;directed mutagenesis;protein;endo-beta-1,3-glucanase;beta-1,3-glucanase;identification;resolution;binding
日期: 2008
Issue Date: 2012-12-07 16:09:34 (UTC+8)
關連: Acta Crystallographica Section D-Biological Crystallography, Volume 64, Page(s) 964-970.
摘要: The catalytic domain structure of Streptomyces sioyaensis-1,3-beta-glucanase (278 amino acids), a member of glycosyl hydrolase family 16 (GHF16), was determined to 1.5 angstrom resolution in space group P2(1)2(1)2(1). The enzyme specifically hydrolyzes the glycosidic bond of the 1,3-beta-linked glucan substrate. The overall structure contains two antiparallel six- and seven-stranded beta-sheets stacked in a beta-sandwich jelly-roll motif similar to the fold of GHF16 1,3-1,4-beta-glucanases. The active-site cleft of the enzyme is distinct, with the closure of one end primarily caused by two protruding loop insertions and two key residues, Tyr38 and Tyr134. The current known structures of 1,3-1,4-beta-glucanases and 1,3-beta-glucanase from Nocardiopsis sp., on the other hand, have open-channel active-site clefts that can accommodate six beta-D-glucopyranosyl units. The active-site structure of 1,3-beta-glucanase was compared with those of other homologous structures in order to address the binding and enzymatic specificity for 1,3-beta-linked glucans in Streptomyces. This information could be helpful in the development of specific antifungal agents.
Relation: Acta Crystallographica Section D-Biological Crystallography
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 李天雄
[依教師分類] 孟孟孝

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