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National Chung Hsing University Institutional Repository - NCHUIR > 生命科學院 > 生物化學研究所 > 依資料類型分類 > 期刊論文 >  An atypical RNA pseudoknot stimulator and an upstream attenuation signal for-1 ribosomal frameshifting of SARS coronavirus

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/130869

標題: An atypical RNA pseudoknot stimulator and an upstream attenuation signal for-1 ribosomal frameshifting of SARS coronavirus
作者: Su, M.C.;Chang, C.T.;Chu, C.H.;Tsai, C.H.;Chang, K.Y.
蔡慶修;張功耀
關鍵字: acute respiratory syndrome;mammary-tumor virus;messenger-rna;retroviral rna;viral-rna;translation;expression;efficiency;kilobases;triplex
日期: 2005
Issue Date: 2012-12-07 16:09:42 (UTC+8)
關連: Nucleic Acids Research, Volume 33, Issue 13, Page(s) 4265-4275.
摘要: The -1 ribosomal frameshifting requires the existence of an in cis RNA slippery sequence and is promoted by a downstream stimulator RNA. An atypical RNA pseudoknot with an extra stem formed by complementary sequences within loop 2 of an H-type pseudoknot is characterized in the severe acute respiratory syndrome coronavirus (SARS CoV) genome. This pseudoknot can serve as an efficient stimulator for -1 frameshifting in vitro. Mutational analysis of the extra stem suggests frameshift efficiency can be modulated via manipulation of the secondary structure within the loop 2 of an infectious bronchitis virus-type pseudoknot. More importantly, an upstream RNA sequence separated by a linker 5' to the slippery site is also identified to be capable of modulating the -1 frameshift efficiency. RNA sequence containing this attenuation element can downregulate -1 frameshifting promoted by an atypical pseudoknot of SARS CoV and two other pseudoknot stimulators. Furthermore, frameshift efficiency can be reduced to half in the presence of the attenuation signal in vivo. Therefore, this in cis RNA attenuator represents a novel negative determinant of general importance for the regulation of -1 frameshift efficiency, and is thus a potential antiviral target.
Relation: Nucleic Acids Research
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 蔡慶修
[依教師分類] 張功耀

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