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National Chung Hsing University Institutional Repository - NCHUIR > 學術研究中心 > 生物科技發展中心 > 依資料類型分類 > 期刊論文 >  Optimization of Ultrasound-Accelerated Synthesis of Enzymatic Octyl Hydroxyphenylpropionate by Response Surface Methodology

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/130915

標題: Optimization of Ultrasound-Accelerated Synthesis of Enzymatic Octyl Hydroxyphenylpropionate by Response Surface Methodology
作者: Lee, C.C.;Chen, H.C.;Ju, H.Y.;Liu, Y.C.;Chen, J.H.;Shie, S.Y.;Chang, C.;Shieh, C.J.
陳建華;劉永銓;謝淳仁
關鍵字: phenolic acid ester;lipase;response surface methodology (RSM);solvent;free;ultrasound;lipase-catalyzed synthesis;phenolic-acids;esters;oil;hydrolysis;media
日期: 2010
Issue Date: 2012-12-07 16:10:53 (UTC+8)
關連: Biotechnology Progress, Volume 26, Issue 6, Page(s) 1629-1634.
摘要: The ultrasound-accelerated enzymatic synthesis of octyl hydroxyphenylpropionate (OHPP) from p-hydroxyphenylpropionic acid (HPPA) and octanol was investigated in this study. A commercially available immobilized lipase from Candida antarctica, Novozym (R) 435, was used as the biocatalyst. A three-level-three-factor Box-Behnken design experiment and response surface methodology were used to evaluate the effects of temperature, reaction time, and enzyme activity on percent yield of OHPP. The results indicated that temperature and enzyme activity significantly affected percent yield, whereas reaction time did not. A model for the synthesis of OHPP was established. Based on a ridge max analysis, the optimum conditions for OHPP synthesis were predicted to use a reaction temperature of 58.8 degrees C, a reaction time of 14.6 h, and an enzyme activity of 410.5 PLU with a yield of 98.5%. A reaction was performed under these optimal conditions, and a yield of 97.5% +/- 0.1% was obtained. (C) 2010 American Institute of Chemical Engineers Biotechnol. Prog., 26: 16291634, 2010
Relation: Biotechnology Progress
Appears in Collections:[依教師分類] 劉永銓
[依資料類型分類] 期刊論文
[依教師分類] 陳建華

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