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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 生物科技學研究所 > 依資料類型分類 > 期刊論文 >  The 3 '-terminal sequence of Bamboo mosaic virus minus-strand RNA interacts with RNA-dependent RNA polymerase and initiates plus-strand RNA synthesis

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/131080

標題: The 3 '-terminal sequence of Bamboo mosaic virus minus-strand RNA interacts with RNA-dependent RNA polymerase and initiates plus-strand RNA synthesis
作者: Chen, I.H.;Lin, J.W.;Chen, Y.J.;Wang, Z.C.;Liang, L.F.;Meng, M.H.;Hsu, Y.H.;Tsai, C.H.
蔡慶修;徐堯煇;孟孟孝
關鍵字: helicase-like domain;potexvirus rna;functional-analysis;in-vitro;3'-untranslated region;replication;guanylyltransferase;accumulation;proteins;promoter
日期: 2010
Issue Date: 2012-12-07 16:16:38 (UTC+8)
關連: Molecular Plant Pathology, Volume 11, Issue 2, Page(s) 203-212.
摘要: A 3'-terminal, 77-nucleotide sequence of Bamboo mosaic virus (BaMV) minus-strand RNA (Ba-77), comprising a 5' stem-loop, a spacer and a 3'-CUUUU sequence, can be used to initiate plus-strand RNA synthesis in vitro. To understand the mechanism of plus-strand RNA synthesis, mutations were introduced in the 5' untranslated region of BaMV RNA, resulting in changes at the 3' end of minus-strand RNA. The results showed that at least three uridylate residues in 3'-CUUUU are required and the changes at the penultimate U are deleterious to viral accumulation in Nicotiana benthamiana protoplasts. Results from UV-crosslinking and in vitro RNA-dependent RNA polymerase competition assays suggested that the replicase preferentially interacts with the stem structure of Ba-77. Finally, CMV/83 + UUUUC, a heterologus RNA, which possesses about 80 nucleotides containing the 3'-CUUUU pentamer terminus, and which folds into a secondary structure similar to that of Ba-77, could be used as template for RNA production by the BaMV replicase complex in vitro.
Relation: Molecular Plant Pathology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 蔡慶修
[依教師分類] 孟孟孝

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