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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 生物科技學研究所 > 依資料類型分類 > 期刊論文 >  The arginine-rich motif of Bamboo mosaic virus satellite RNA-encoded P20 mediates self-interaction, intracellular targeting, and cell-to-cell movement

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/131162

標題: The arginine-rich motif of Bamboo mosaic virus satellite RNA-encoded P20 mediates self-interaction, intracellular targeting, and cell-to-cell movement
作者: Palani, P.V.;Kasiviswanathan, V.;Chen, J.C.F.;Chen, W.;Hsu, Y.H.;Lin, N.S.
徐堯煇
關鍵字: protein-protein interaction;triple-gene-block;yellow-vein-virus;binding domain;subcellular-localization;nucleotide-sequence;capsid protein;viral-rna;potexvirus;replication;plasmodesmata
日期: 2006
Issue Date: 2012-12-07 16:18:37 (UTC+8)
關連: Molecular Plant-Microbe Interactions, Volume 19, Issue 7, Page(s) 758-767.
摘要: Satellite RNA of Bamboo mosaic virus (satBaMV) has a single open reading frame for a nonstructural, RNA-binding protein, P20, which facilitates the long-distance movement of satBaMV in Nicotiana benthamiana. Here, we elucidate various biological properties of P20 and the involvement of a single domain in its activities. P20 displayed a strong self-interaction in vitro and in vivo, and cross-linking assays demonstrated its oligomerization. Domain mapping, using the bacterial two-hybrid system, indicated that the self-interacting domain overlaps the RNA-binding domain in the N-terminal arginine-rich motif (ARM) of P20. The deletion of the ARM abolished the self-interaction of P20 in vitro and in vivo and impaired its intracellular targeting and efficient cell-to-cell movement in N. benthamiana leaves. Moreover, RNA and protein accumulation of the ARM deletion mutant of satBaMV was significantly reduced in leaves systemically coinfected with Bamboo mosaic potexvirus and satBaMV. This is the first report of the involvement of ARM in various biological activities of a satellite RNA-encoded protein during infection of its host.
Relation: Molecular Plant-Microbe Interactions
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 徐堯煇

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