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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 生物產業機電工程學系 > 依資料類型分類 > 期刊論文 >  Deregulation of AP-1 proteins in collagen gel-induced epithelial cell apoptosis mediated by low substratum rigidity

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/131333

標題: Deregulation of AP-1 proteins in collagen gel-induced epithelial cell apoptosis mediated by low substratum rigidity
作者: Wang, Y.H.;Chiu, W.T.;Wang, Y.K.;Wu, C.C.;Chen, T.L.;Teng, C.F.;Chang, W.T.;Chang, H.C.;Tang, M.J.
吳靖宙
關鍵字: canine kidney-cells;jun activation domain;c-jun;extracellular-matrix;terminal phosphorylation;fibronectin deposition;mechanical-properties;signal-transduction;focal adhesions;down-regulation
日期: 2007
Issue Date: 2012-12-07 16:22:42 (UTC+8)
關連: Journal of Biological Chemistry, Volume 282, Issue 1, Page(s) 752-763.
摘要: In this study, we established that collagen gel, but not collagen gel coating, induced apoptosis exclusively in epithelial cell lines, which indicated that low substratum rigidity might trigger cell apoptosis. To confirm this, we used collagen gels with different rigidities due to cross-linking or physical disruption of collagen fibrils caused by sonication. We found that collagen gel-induced apoptosis was inversely correlated with substratum rigidity. Low substratum rigidity collagen gel-induced apoptosis was neither prevented by Bcl-2 overexpression nor preceded by mitochondrial release of cytochrome c. This suggested that the mitochondrial pathway was not involved in low substratum rigidity-induced apoptosis. Low substratum rigidity activated c-jun N-terminal kinase (JNK) within 4 h, but it also rapidly down-regulated c-jun within I h and triggered persistent aberrant expression of c-Fos for at least 24 h. Either reduced c-jun expression or c-Fos overexpression induced apoptosis in several epithelial cells. Inhibiting low substratum rigidity-induced JNK activation prevented aberrant c-Fos expression but only partially blocked low substratum rigidity-induced apoptosis. Taking these results together, we conclude that low substratum rigidity collagen gel induced apoptosis in epithelial cells and that deregulated AP-1 proteins mediated that apoptosis, at least in part.
Relation: Journal of Biological Chemistry
Appears in Collections:[依教師分類] 張厚謙
[依資料類型分類] 期刊論文
[依教師分類] 吳靖宙
[依教師分類] 吳靖宙
[依教師分類] 張厚謙

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