English  |  正體中文  |  简体中文  |  Items with full text/Total items : 43312/67235
Visitors : 2094875      Online Users : 8
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU/NCHU Library IR team.
National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 昆蟲學系 > 依資料類型分類 > 期刊論文 >  Amplification of single bulb mites by nested PCR: Species-specific primers to detect Rhizoglyphus robini and R. setosus (Acari: Acaridae)

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/132534

標題: Amplification of single bulb mites by nested PCR: Species-specific primers to detect Rhizoglyphus robini and R. setosus (Acari: Acaridae)
作者: Li, H.Y.;Hua, T.;Yeh, W.B.
葉文斌
關鍵字: Acaridae;Nested PCR;Bulb mite;Ribosomal DNA;Molecular identification;whole-genome amplification;ribosomal dna-sequences;phylogenetic-relationships;mitochondrial-dna;tetranychidae;identification;rhinonyssidae;inference;complex;markers
日期: 2010
Issue Date: 2012-12-07 17:03:39 (UTC+8)
關連: Journal of Asia-Pacific Entomology, Volume 13, Issue 4, Page(s) 267-271.
摘要: A major problem of using molecular amplicons for DNA amplification in mite systematics is that sufficient template cannot always be acquired from an individual mite. To solve this problem, we developed a nested PCR for DNA amplification of single Rhizoglyphus robini and R. setosus bulb mites. A dilution up to 10(5) of the DNA from a single egg, larva, nymph or adult contained enough templates for amplification of the target ribosomal region. However, the use of specific primers in the second PCR is necessary to reduce the generation of non-target DNAs from symbiotic organisms. Identification of bulb mites collected from seven sampling locations in Taiwan or of bulb mites that were used in simulated experiments in the presence of host plant tissues was unambiguous with specific PCR primers. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2010 Published by Elsevier B.V. All rights reserved.
Relation: Journal of Asia-Pacific Entomology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 葉文斌

loading Web of Knowledge data....

Files in This Item:

File SizeFormat
index.html0KbHTML363View/Open


 


學術資源

著作權聲明

本網站為收錄中興大學學術著作及學術產出,已積極向著作權人取得全文授權,並盡力防止侵害著作權人之權益。如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員,將盡速為您處理。

本網站之數位內容為國立中興大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用。

聯絡網站維護人員:wyhuang@nchu.edu.tw,04-22840290 # 412。

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU/NCHU Library IR team Copyright ©   - Feedback