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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 昆蟲學系 > 依資料類型分類 > 期刊論文 >  Amplification of single bulb mites by nested PCR: Species-specific primers to detect Rhizoglyphus robini and R. setosus (Acari: Acaridae)

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標題: Amplification of single bulb mites by nested PCR: Species-specific primers to detect Rhizoglyphus robini and R. setosus (Acari: Acaridae)
作者: Li, H.Y.;Hua, T.;Yeh, W.B.
關鍵字: Acaridae;Nested PCR;Bulb mite;Ribosomal DNA;Molecular identification;whole-genome amplification;ribosomal dna-sequences;phylogenetic-relationships;mitochondrial-dna;tetranychidae;identification;rhinonyssidae;inference;complex;markers
日期: 2010
Issue Date: 2012-12-07 17:03:39 (UTC+8)
關連: Journal of Asia-Pacific Entomology, Volume 13, Issue 4, Page(s) 267-271.
摘要: A major problem of using molecular amplicons for DNA amplification in mite systematics is that sufficient template cannot always be acquired from an individual mite. To solve this problem, we developed a nested PCR for DNA amplification of single Rhizoglyphus robini and R. setosus bulb mites. A dilution up to 10(5) of the DNA from a single egg, larva, nymph or adult contained enough templates for amplification of the target ribosomal region. However, the use of specific primers in the second PCR is necessary to reduce the generation of non-target DNAs from symbiotic organisms. Identification of bulb mites collected from seven sampling locations in Taiwan or of bulb mites that were used in simulated experiments in the presence of host plant tissues was unambiguous with specific PCR primers. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2010 Published by Elsevier B.V. All rights reserved.
Relation: Journal of Asia-Pacific Entomology
Appears in Collections:[依資料類型分類] 期刊論文
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