English  |  正體中文  |  简体中文  |  Items with full text/Total items : 43312/67235
Visitors : 2107241      Online Users : 10
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU/NCHU Library IR team.
National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 食品暨應用生物科技學系 > 依資料類型分類 > 期刊論文 >  Adherent Properties and Macrophage Activation Ability of 3 Strains of Lactic Acid Bacteria

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/132748

標題: Adherent Properties and Macrophage Activation Ability of 3 Strains of Lactic Acid Bacteria
作者: Lin, W.H.;Wu, C.R.;Fang, T.J.;Lee, M.S.;Lin, K.L.;Chen, H.C.;Huang, S.Y.;Hseu, Y.C.
方 繼
關鍵字: adhesion;lactic acid bacteria;macrophage;RAW 264.7;TNF-alpha;intestinal epithelial-cells;lactobacillus-rhamnosus gg;escherichia-coli o157-h7;acidophilus lafti l10;necrosis-factor-alpha;human colonic tissue;antagonistic activity;probiotic strains;immune-response;adhesion properties
日期: 2011
Issue Date: 2012-12-14 09:23:15 (UTC+8)
關連: Journal of Food Science, Volume 76, Issue 1, Page(s) M1-M7.
摘要: Lactic acid bacteria (LAB) must possess probiotic properties in order to be beneficial to humans and animals. The adherent properties, the acid and bile tolerance as well as the macrophage activation ability of isolated LAB strains were investigated in this study. The adhesion was analyzed following heat, acid, trypsin, and sodium periodate treatments. Production of the cytokine tumor necrosis factor-alpha (TNF-alpha) by RAW 264.7 macrophages was also measured after stimulation with heat-killed LAB strains. The viable strains of Lactobacillus fermentum AF7, L. acidophilus GG5, and L. plantarum BB9 were able to tightly adhere to the intestinal Caco-2 cells. In addition, the GG5 strain was not affected by heating, acid, trypsin, or sodium periodate treatment. However, the adhesion of strains AF7 and BB9 was reduced significantly by heating and trypsin treatment. This result suggested the GG5 and AF7 or BB9 strains had different cell-surface adherent factors. TNF-alpha production by the RAW 264.7 macrophages was induced significantly following stimulation with heat-killed LAB at 10(8) CFU/mL in a dose-dependent fashion. In addition, macrophage activity was similar whether the treatment consisted of live probiotics, or probiotics treated with heat, acid, or trypsin. However, the activity was reduced after treating with sonication. These in vitro results showed that the LAB studied possess probiotic characteristics, such as acid or bile tolerance, adherent capability, and immune activation, and may suggest that these LAB strains retain their probiotic activity as they pass through the gastrointestinal tract.
Relation: Journal of Food Science
Appears in Collections:[依資料類型分類] 期刊論文

loading Web of Knowledge data....

Files in This Item:

File SizeFormat
index.html0KbHTML406View/Open


 


學術資源

著作權聲明

本網站為收錄中興大學學術著作及學術產出,已積極向著作權人取得全文授權,並盡力防止侵害著作權人之權益。如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員,將盡速為您處理。

本網站之數位內容為國立中興大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用。

聯絡網站維護人員:wyhuang@nchu.edu.tw,04-22840290 # 412。

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU/NCHU Library IR team Copyright ©   - Feedback