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National Chung Hsing University Institutional Repository - NCHUIR > 獸醫學院 > 微生物暨公共衛生學研究所 > 依資料類型分類 > 期刊論文 >  Comparative analysis of full genomic sequences among different genotypes of dengue virus type 3

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/134017

標題: Comparative analysis of full genomic sequences among different genotypes of dengue virus type 3
作者: King, C.C.;Chao, D.Y.;Chien, L.J.;Chang, G.J.J.;Lin, T.H.;Wu, Y.C.;Huang, J.H.
趙黛瑜
關鍵字: hemorrhagic-fever;phylogenetic analysis;epidemic;emergence;infections;inhibition;serotype-3;alignment;thailand;strains
日期: 2008
Issue Date: 2012-12-14 10:26:47 (UTC+8)
關連: Virology Journal, Volume 5.
摘要: Background: Although the previous study demonstrated the envelope protein of dengue viruses is under purifying selection pressure, little is known about the genetic differences of full-length viral genomes of DENV-3. In our study, complete genomic sequencing of DENV-3 strains collected from different geographical locations and isolation years were determined and the sequence diversity as well as selection pressure sites in the DENV genome other than within the E gene were also analyzed. Results: Using maximum likelihood and Bayesian approaches, our phylogenetic analysis revealed that the Taiwan's indigenous DENV-3 isolated from 1994 and 1998 dengue/DHF epidemics and one 1999 sporadic case were of the three different genotypes -I, II, and III, each associated with DENV-3 circulating in Indonesia, Thailand and Sri Lanka, respectively. Sequence diversity and selection pressure of different genomic regions among DENV-3 different genotypes was further examined to understand the global DENV-3 evolution. The highest nucleotide sequence diversity among the fully sequenced DENV-3 strains was found in the nonstructural protein 2A ( mean +/- SD: 5.84 +/- 0.54) and envelope protein gene regions ( mean +/- SD: 5.04 +/- 0.32). Further analysis found that positive selection pressure of DENV-3 may occur in the non-structural protein 1 gene region and the positive selection site was detected at position 178 of the NS1 gene. Conclusion: Our study confirmed that the envelope protein is under purifying selection pressure although it presented higher sequence diversity. The detection of positive selection pressure in the non-structural protein along genotype II indicated that DENV-3 originated from Southeast Asia needs to monitor the emergence of DENV strains with epidemic potential for better epidemic prevention and vaccine development.
Relation: Virology Journal
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 趙黛瑜

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