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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 農業暨自然資源學院 > 依資料類型分類 > 期刊論文 >  Inhibition of soil-borne plant pathogens by the treatment of sinigrin and myrosinases released from reconstructed Escherichia coli and Pichia pastoris

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/134587

標題: Inhibition of soil-borne plant pathogens by the treatment of sinigrin and myrosinases released from reconstructed Escherichia coli and Pichia pastoris
作者: Chung, W.C.;Huang, H.C.;Chiang, B.T.;Huang, J.W.
黃振文
關鍵字: Escherichia coli;Pichia pastoris;myrosinase genes TGG1 and TGG2;Arabidopsis thaliana;sinigrin;allyl isothiocyanate;Rhizoctonia solani;brassica-napus;gene family;arabidopsis-thaliana;expression;organization;evolution;products;seed
日期: 2005
Issue Date: 2012-12-14 10:57:36 (UTC+8)
關連: Biocontrol Science and Technology, Volume 15, Issue 5, Page(s) 455-465.
摘要: Myrosinases ( thioglucoside glucohydrolase, EC 3.2.3.1) are able to hydrolyse glucosinolates in natural plant products. In Arabidopsis thaliana three different genes with different tissue- specific expressions and distribution patterns encode myrosinases. cDNAs of myrosinase genes ( TGG1 and TGG2) were isolated from A. thaliana and expressed in Escherichia coli and Pichia pastoris. The enzyme activities of myrosinase TGG1 and TGG2 genes expressed in P. pastoris were higher than those expressed in E. coli. Among six glucosinolates tested for specificity to myrosinases TGG1 and TGG2, the suitable substrates for these two genes expressed in P. pastoris and E. coli were sinigrin, gluconapin, glucobrassicanapin and glucoraphanin. Treatment of sinigrin with myrosinases excreted from reconstructed E. coli and P. pastoris with TGG1 and TGG2 genes showed strong fungicidal effects on mycelial growth of Rhizoctonia solani AG- 4, Sclerotium rolfsii, and Pythium aphanidermatum. This study suggests that the combination of glucosinolate with myrosinases excreted from the reconstructed microbes may be of potential for control of soil- borne diseases.
Relation: Biocontrol Science and Technology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 黃振文

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