English  |  正體中文  |  简体中文  |  Items with full text/Total items : 43312/67235
Visitors : 2049841      Online Users : 2
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU/NCHU Library IR team.
National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 農業暨自然資源學院 > 依資料類型分類 > 期刊論文 >  Development of a semiselective medium for detection of Mycosphaerella pinodes in soil, plant debris and seed

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/134590

標題: Development of a semiselective medium for detection of Mycosphaerella pinodes in soil, plant debris and seed
作者: Chen, M.H.;Chung, W.C.;Huang, H.C.;Huang, J.W.
黃振文
關鍵字: garden pea;leaf blight;Mycosphaerella pinodes;SC-semiselective medium;pea pisum-sativum;yield components;field peas;ascochyta;survival;paraquat;fungi
日期: 2010
Issue Date: 2012-12-14 10:57:46 (UTC+8)
關連: Canadian Journal of Plant Pathology-Revue Canadienne De Phytopathologie, Volume 32, Issue 3, Page(s) 342-350.
摘要: Garden pea leaf blight, caused by Mycosphaerella pinodes, is a severe disease of peas worldwide. Detection of this pathogen from seed, plant debris and soil is difficult using non-selective agar media. This study was conducted to develop a semiselective medium for the isolation of this pathogen. Of the 14 carbon sources and 18 nitrogen sources tested, maltose and soluble starch were the most suitable carbon sources and casein was the most important nitrogen source for mycelial growth of three isolates of M. pinodes. Among 13 pesticides and two antibiotics tested, atrazine, mepronil, paraquat, propamocarb and TMTD showed no adverse effect on growth of M. pinodes. Based on these results, a starch-casein semiselective medium (designated as the SC-semiselective medium) was developed by amendment of 1 L Czapek-Dox agar with 30 g soluble starch, 2 g casein, 25 g mL-1 propamocarb, 10 g mL-1 benomyl, 50 g mL-1 mepronil, 300 g mL-1 atrazine, 100 g mL-1 streptomycin sulfate and 200 g mL-1 neomycin sulfate. This SC-semiselective medium was effective for detection of M. pinodes in artificially and naturally infested field soils, diseased seeds, leaves, and stems of peas. It should have potential for applications in research on biology, ecology and control of M. pinodes in peas.
Relation: Canadian Journal of Plant Pathology-Revue Canadienne De Phytopathologie
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 黃振文

loading Web of Knowledge data....

Files in This Item:

File SizeFormat
index.html0KbHTML305View/Open


 


學術資源

著作權聲明

本網站為收錄中興大學學術著作及學術產出,已積極向著作權人取得全文授權,並盡力防止侵害著作權人之權益。如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員,將盡速為您處理。

本網站之數位內容為國立中興大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用。

聯絡網站維護人員:wyhuang@nchu.edu.tw,04-22840290 # 412。

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU/NCHU Library IR team Copyright ©   - Feedback