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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 農業暨自然資源學院 > 依資料類型分類 > 期刊論文 >  Evaluation of onion cultivars for resistance to Aspergillus niger, the causal agent of black mold

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/134592

標題: Evaluation of onion cultivars for resistance to Aspergillus niger, the causal agent of black mold
作者: Ko, S.S.;Huang, J.W.;Wang, J.F.;Shanmugasundaram, S.;Chang, W.N.
關鍵字: Allium cepa;disease resistance;inoculation method
日期: 2002
Issue Date: 2012-12-14 10:57:49 (UTC+8)
關連: Journal of the American Society for Horticultural Science, Volume 127, Issue 4, Page(s) 697-702.
摘要: Black mold (BM), caused by Aspergillus niger Tiegh., is a common onion (Allium cepa L.) postharvest disease under hot and humid storage conditions. This study aimed to develop a BM screening protocol, to evaluate onion cultivars; for resistance. The effects of pathogen isolate, inoculum density, incubation temperature, and inoculation method on severity of BM were investigated in designing the screening protocol. Variation in virulence was observed and two highly virulent subcultures, An4-2 and An39-3, were selected for study. Disease severity, both in lesion size and sporulation degree, increased with higher inoculum densities and incubation temperatures. In contrast, small lesions and no sporulation were observed when onion scales were inoculated with 1 X 104 Spores/mL or incubated at temperatures lower than 20degreesC. Inoculation of onion scales or entire bulbs were compared and scale inoculation correlated better with BM incidence in storage. The procedure for BM screening protocol included: wound inoculation of onion slices with 40 muL inoculum suspension of 1 x 10(-5) spores/mL, incubation at 25degreesC with 12 hour photoperiod, and rating lesion diameter and sporulation 4 days after inoculation. Using this screening protocol, 42 onion cultivars were evaluated and cultivars with better storability such as 'Red Pinoy', 'Serrana', 'Dehydrator No.3', and 'Moonlight' were tolerant to A. niger. These cultivars showed small lesions and no sporulation during screening. On the other hand, cultivars with poor storability like 'Texas Early Grano 502', 'Granex 429', and 'Explorer' exhibited larger lesions with profuse sporulation. Lesion diameter caused by the scale inoculation correlated significantly with bulb storage loss (r = 0.51, P < 0.01) as well as with bulb dry matter (r = -0.48, P < 0.01) and total soluble solids (r = 0.51, P < 0.01). The screening protocol and tolerant materials are now successfully used in the Asian Vegetable Research and Development Center onion breeding program for resistance to black mold.
Relation: Journal of the American Society for Horticultural Science
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 黃振文

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