English  |  正體中文  |  简体中文  |  Items with full text/Total items : 43312/67235
Visitors : 2052206      Online Users : 9
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU/NCHU Library IR team.
National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 農業暨自然資源學院 > 依資料類型分類 > 期刊論文 >  Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp niveum in Taiwan

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/134596

標題: Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp niveum in Taiwan
作者: Lin, Y.H.;Chen, K.S.;Chang, J.Y.;Wan, Y.L.;Hsu, C.C.;Huang, J.W.;Chang, P.F.L.
黃振文
關鍵字: formae-speciales;pcr assays;race;wilt;resistance;watermelon;culmorum;gene;graminearum;avenaceum
日期: 2010
Issue Date: 2012-12-14 10:57:56 (UTC+8)
關連: New Biotechnology, Volume 27, Issue 4, Page(s) 409-418.
摘要: Fusarium wilt, caused by Fusarium oxysporum (Fo), is one of the most important fungal diseases worldwide. Like other plant pathogens, Fo displays specialized forms in association with its hosts. For example, F. oxysporum f. sp. niveum (Fon) is the damaging pathogen causing Fusarium wilt disease on watermelon, whereas F. oxysporum f. sp. cubense is the pathogen that infects banana. A rapid and reliable pathogen identification or disease diagnosis is essential for the integrated disease management practices in many crops. In this study, two new primer sets, Fon-1/Fon-2 and FnSc-1/FnSc-2, were developed to differentiate Fon and Fo, respectively. The PCR method using the novel primer sets has high sensitivity to detect Fon when the DNA concentration was as low as 0.01 pg or when the conidia number was as few as 5. In comparison with the published primer set, the Fon-1/Fon-2 primer set, derived from the sequence of OP-M12 random primer-amplified fragment, produced a 174 bp DNA fragment, and was more specific to Fon in Taiwan. In addition, with optimized PCR parameters, the molecular method using the Fon-1/Fon-2 primer set could directly detect Fon even when watermelon samples were collected in its early stage of disease development.
Relation: New Biotechnology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 黃振文

Files in This Item:

There are no files associated with this item.



 


學術資源

著作權聲明

本網站為收錄中興大學學術著作及學術產出,已積極向著作權人取得全文授權,並盡力防止侵害著作權人之權益。如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員,將盡速為您處理。

本網站之數位內容為國立中興大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用。

聯絡網站維護人員:wyhuang@nchu.edu.tw,04-22840290 # 412。

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU/NCHU Library IR team Copyright ©   - Feedback