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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 農業暨自然資源學院 > 依資料類型分類 > 期刊論文 >  Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp niveum in Taiwan

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/134596

標題: Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp niveum in Taiwan
作者: Lin, Y.H.;Chen, K.S.;Chang, J.Y.;Wan, Y.L.;Hsu, C.C.;Huang, J.W.;Chang, P.F.L.
關鍵字: formae-speciales;pcr assays;race;wilt;resistance;watermelon;culmorum;gene;graminearum;avenaceum
日期: 2010
Issue Date: 2012-12-14 10:57:56 (UTC+8)
關連: New Biotechnology, Volume 27, Issue 4, Page(s) 409-418.
摘要: Fusarium wilt, caused by Fusarium oxysporum (Fo), is one of the most important fungal diseases worldwide. Like other plant pathogens, Fo displays specialized forms in association with its hosts. For example, F. oxysporum f. sp. niveum (Fon) is the damaging pathogen causing Fusarium wilt disease on watermelon, whereas F. oxysporum f. sp. cubense is the pathogen that infects banana. A rapid and reliable pathogen identification or disease diagnosis is essential for the integrated disease management practices in many crops. In this study, two new primer sets, Fon-1/Fon-2 and FnSc-1/FnSc-2, were developed to differentiate Fon and Fo, respectively. The PCR method using the novel primer sets has high sensitivity to detect Fon when the DNA concentration was as low as 0.01 pg or when the conidia number was as few as 5. In comparison with the published primer set, the Fon-1/Fon-2 primer set, derived from the sequence of OP-M12 random primer-amplified fragment, produced a 174 bp DNA fragment, and was more specific to Fon in Taiwan. In addition, with optimized PCR parameters, the molecular method using the Fon-1/Fon-2 primer set could directly detect Fon even when watermelon samples were collected in its early stage of disease development.
Relation: New Biotechnology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 黃振文

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