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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 農業暨自然資源學院 > 依資料類型分類 > 期刊論文 >  Specific detection of benzimidazole resistance in Colletotrichum gloeosporioides from fruit crops by PCR-RFLP

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/134597

標題: Specific detection of benzimidazole resistance in Colletotrichum gloeosporioides from fruit crops by PCR-RFLP
作者: Chung, W.H.;Chung, W.C.;Peng, M.T.;Yang, H.R.;Huang, J.W.
黃振文
關鍵字: beta-tubulin gene;benomyl-resistance;botrytis-cinerea;field strains;mycosphaerella-fijiensis;aspergillus-nidulans;venturia-inaequalis;identification;sensitivity;fungicides
日期: 2010
Issue Date: 2012-12-14 10:57:57 (UTC+8)
關連: New Biotechnology, Volume 27, Issue 1, Page(s) 17-24.
摘要: Anthracnose diseases, caused by Colletotrichum gloeosporioides, are a worldwide problem and are especially important in Taiwan owing to the severe economic damage they cause to tropical fruits that are grown for local consumption and export. Benzimidazoles are systemic fungicides widely used for controlling these diseases in Taiwan. Thirty-one isolates of C. gloeosporioides from mango and strawberry grown in Taiwan were examined for their sensitivity to benzimidazole fungicides. The responses of the isolates grown on benzimidazole-amended culture media were characterized as sensitive, moderately resistant, resistant or highly resistant. Analysis of point mutations in the beta-tubulin gene by DNA sequencing of PCR-amplified fragments revealed a substitution of GCG for GAG at codon 198 in resistant and highly resistant isolates and a substitution of TAG for TTC at codon 200 in moderately resistant isolates. A set of specific primers, TubGF1 and TubGR, was designed to amplify a portion of the B-tubulin gene for the detection of benzimidazole-resistant C. gloeosporioides. Bsh1236I restriction maps of the amplified beta-tubulin gene showed that the resistant isolate sequence, but not the sensitive isolate sequence, was cut. The PCR restriction fragment length polymorphism (PCR-RFLP) was validated to detect benzimidazole-resistant and benzimidazole-sensitive C. gloeosporioides isolates recovered from avocado, banana, carambola, dragon fruit, grape, guava, jujube, lychee, papaya, passion fruit and wax apple. This method has the potential to become a valuable tool for monitoring the occurrence of benzimidazole-resistant C. gloeosporioides and for assessment of the need for alternative management practices.
Relation: New Biotechnology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 黃振文

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