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標題: Biological and biochemical detection techniques for glufosinate
作者: Zhou, X.H.;Wang, C.Y.
王慶裕
關鍵字: bioassay;biochemical marker;herbicide detection;herbicide persistence;glutamine-synthetase activity;ammonium accumulation;liquid-chromatography;rapid-determination;zea-mays;phosphinothricin;photosynthesis;glyphosate;growth;acid
日期: 2006
Issue Date: 2012-12-14 11:00:00 (UTC+8)
關連: Weed Science, Volume 54, Issue 3, Page(s) 413-418.
摘要: Biological and biochemical methods, based on glufosinate inhibitory effects on plant growth and nitrogen metabolism, were examined for their applications to detect this herbicide. Dose-response analysis of radicle growth inhibition showed that, among six vegetables tested, Chinese mustard and edible amaranth were the most sensitive to glufosinate. Field mustard and cruciferous Ching-Geeng were found to be more sensitive to this herbicide than the other four vegetables when three-leaf-seedlings were tested in another bioassay. In three-leaf seedlings of Ching-Geeng, accumulation of ammonium, a biochemical marker for glufosinate toxicity because of its inhibition of glutamine synthetase, showed a linear regression to the log-transformed concentrations of glufosinate ranging from 7.5 X 10(-5) to 1.5 X 10(-3) M. For the detection of glufosinate lower than 7.5 X 10(-6) M, a linear regression was observed between ammonium accumulation and the applied concentration, instead of the log-trans- formed value, of glufosinate. A similar relationship was observed between the accumulation in Ching-Geeng seedlings of glyoxylate, another biochemical marker, and glufosinate but with a narrower range than that for ammonium accumulation. The applicability of ammonium accumulation in three-leaf seedlings of Ching-Geeng to detect glufosinate residue in water and soil was confirmed by high-performance liquid chromatography (HPLC).
Relation: Weed Science
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 王慶裕

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