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National Chung Hsing University Institutional Repository - NCHUIR > 農業暨自然資源學院 > 農藝學系 > 依資料類型分類 > 期刊論文 >  Cloning and characterization of maize B chromosome sequences derived from microdissection

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/134642

標題: Cloning and characterization of maize B chromosome sequences derived from microdissection
作者: Cheng, Y.M.;Lin, B.Y.
鄭雅銘
關鍵字: knob heterochromatin;molecular analysis;dna;family;translocations;identification;centromere;genes;arms
日期: 2003
Issue Date: 2012-12-14 11:00:46 (UTC+8)
關連: Genetics, Volume 164, Issue 1, Page(s) 299-310.
摘要: Isolation of sequences from the maize B chromosome is always]tampered by its high homology with the normal complements. In this study, this handicap was overcome by cloning the sequences from the pachytene B chromosomes dissected out of a slide by a micromanipulator followed by degenerate oligonucleotide-primed PCR. The isolated sequences were found to hybridize with genomic DNA in a B-dosage-dependent manner and with the pachytene B chromosome by fluorescence in situ hybridization (FISH), corroborating their B origin. A total of 19 B sequences were isolated, all of which are repetitive and, with one exception, are homologous to the A chromosome(s). Three sequences have strong homology to maize sequences that include two knob repeats and one zein gene (noncoding region), and 10 others are homologous to the noncoding region of Adh1, Bz1, Gag, Zein, and B centromere to a lesser degree. Six sequences have no homology to any gene. In addition to FISH, the B-specific sequence and a partially B-specific one were also mapped, by seven newly characterized TB-10L translocations, to a similar location on the central portion of the distal heterochromatic region, spreading over a region of about one-third of the B chromosome.
Relation: Genetics
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 鄭雅銘

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