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National Chung Hsing University Institutional Repository - NCHUIR > 獸醫學院 > 獸醫學院 > 依資料類型分類 > 期刊論文 >  Antibacterial activity of short hydrophobic and basic-rich peptides

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/135702

標題: Antibacterial activity of short hydrophobic and basic-rich peptides
作者: Chen, P.W.;Shyu, C.L.;Mao, F.C.
毛嘉洪
關鍵字: 15-residue lactoferricin derivatives;n-terminal region;antimicrobial;peptide;bovine lactoferrin;tryptophan-rich;binding;protein
日期: 2003
Issue Date: 2012-12-14 12:10:06 (UTC+8)
關連: American Journal of Veterinary Research, Volume 64, Issue 9, Page(s) 1088-1092.
摘要: Objective-To design short and potent analogs of bovine lactoferricin by use of the concepts of lipophilic bulk and cationic charge. Sample Population-5 synthetic peptides of bovine lactoferricin. Procedure-Antibacterial peptides were constructed by synthesizing several decapeptides rich in arginine and tryptophan. Basic residues of bovine lactoferricin (bLf 20-29; residues 20 to 29) were modified by substitution with arginine or lysine and nonbasic residues were modified by substitution with tryptophan, phenylalanine, or isoleucine. Synthetic peptides of bovine lactoferrin (LFB) were designated as LFB-RW (RRWWWRWRRW), LFB-KW (KKWWWKWKKW), LFB-RWa (RRWWRRWRRW), LFB-RF (RRFFFRFRRF), and LFB-RI (RRIIIRWRRI), where R, K, W, F, and I stand for arginine, lysine, tryptophan, phenylalanine, and isoleucine, respectively. Peptides were evaluated by determining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. Results-LFB-RW, LFB-KW, and LFB-RWa possessed equivalent potency as bLf 20-29 against E coli. LFB-RW and LFB-RWa had a 2-fold increase in growth-inhibitory and bactericidal activity against S aureus, compared with bLf 20-29. LFB-RI had the lowest MIC value against E coli among the peptides but lost bactericidal activity. LFB-RW and LFB-KW had stronger bactericidal activities against S aureus or E faecalis, respectively, as well as E coli than the other synthetic peptides. LFB-RF also had antibacterial activity, but this was 2-fold less than that of LFB-RW, as determined by MIC and MBC values. Conclusions and Clinical Relevance-In construction of potent antibacterial peptides, inclusion of arginine, lysine, tryptophan, or isoleucine residues enhances effectiveness against certain bacteria, as measured by MIC or MBC values.
Relation: American Journal of Veterinary Research
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 毛嘉洪

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