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National Chung Hsing University Institutional Repository - NCHUIR > 獸醫學院 > 獸醫病理生物學所 > 依資料類型分類 > 期刊論文 >  Expression of the porcine circovirus type 2 capsid protein subunits and application to an indirect ELISA

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/136026

標題: Expression of the porcine circovirus type 2 capsid protein subunits and application to an indirect ELISA
作者: Wu, P.C.;Chien, M.S.;Tseng, Y.Y.;Lin, J.;Lin, W.L.;Yang, C.Y.;Huang, C.
黃千衿;簡茂盛
關鍵字: porcine circovirus;capsid protein;recombinant subunit;ELISA;multisystemic wasting syndrome;orf2 protein;nuclear-localization;pigs;pcv2;porcine-circovirus-2;identification;infections;antibodies;epitope
日期: 2008
Issue Date: 2012-12-14 13:18:10 (UTC+8)
關連: Journal of Biotechnology, Volume 133, Issue 1, Page(s) 58-64.
摘要: Porcine circovirus type 2 (PCV2) is considered to be associated with post-weaning multisystemic wasting syndrome (PMWS), which is a newly emerged economically important swine disease. The entire coding region of open reading frame 2 (ORF2), encoding the viral capsid protein (Cap), of PCV2 was cloned and sequenced from the clinical specimen obtained from PMWS-affected piglets. Six recombinant subunits, A-F, spanning the defined regions of Cap were produced by E. coli expression system and used as antigens for testing their reactivities with swine sera in the indirect ELISA. The recombinant Cap subunit-based ELISA was evaluated by examining a panel of 12 PCV2-negative and 26 PCV2-positive sera. When the positive/negative cut-off value was set at the mean value of negative sera plus 3 standard deviations, all subunits-based ELISA demonstrated 100% specificities. The N-terminal subunits, A and B, revealed poor reactivity with positive swine sera, whereas, greater immunoreactivity was observed for the C-terminal subunits of which subunits C and D demonstrated good sensitivities of 96.2% and 84.6%, respectively. The recombinant Cap subunits possessing defined antigenicity are easy to produce and the subunit-based ELISA was developed with a high specificity and sensitivity that may provide a useful method for routine serodiagnosis of PCV2 infection. (C) 2007 Elsevier B.V. All rights reserved.
Relation: Journal of Biotechnology
Appears in Collections:[依教師分類] 楊清淵
[依教師分類] 林維亮
[依資料類型分類] 期刊論文
[依教師分類] 簡茂盛
[依教師分類] 黃千衿
[依教師分類] 簡茂盛

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