English  |  正體中文  |  简体中文  |  Items with full text/Total items : 43312/67235
Visitors : 2049842      Online Users : 3
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU/NCHU Library IR team.
National Chung Hsing University Institutional Repository - NCHUIR > 獸醫學院 > 獸醫病理生物學所 > 依資料類型分類 > 期刊論文 >  Cartap-induced cytotoxicity in mouse C2C12 myoblast cell line and the roles of calcium ion and oxidative stress on the toxic effects

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/136105

標題: Cartap-induced cytotoxicity in mouse C2C12 myoblast cell line and the roles of calcium ion and oxidative stress on the toxic effects
作者: Liao, J.W.;Kang, J.J.;Jeng, C.R.;Chang, S.K.;Kuo, M.J.;Wang, S.C.;Liu, M.R.S.;Pang, V.F.
廖俊旺
關鍵字: cartap;cytotoxicity;oxidative stress;Ca2+;C2C12 cells;in vitro;model;skeletal-muscle;diaphragm muscle;generation;injury;mitochondrial;expression;memantine;viability;peroxide;exposure
日期: 2006
Issue Date: 2012-12-14 13:36:34 (UTC+8)
關連: Toxicology, Volume 219, Issue 1-3, Page(s) 73-84.
摘要: Our previous study has demonstrated that instead of neuromuscular blockage cartap, an orgationitrogen insecticide, could cause a marked irreversible Ca2+-dependent contracture in both isolated mouse and rabbit phrenic nerve-diaphragms. We further examined the potential of direct myocytotoxicity of cartap and the possible roles of calcium ion and oxidative stress on cartap-induced muscle cell injury using the mouse myoblast cell line, C2C12. Cartap exerted a dose- and time-dependent cytotoxic effect in C2C12 cells measured by MTT colorimetric assay and trypan blue dye exclusion. The extracellular activities of both creatine kinase (CK) and lactate dehydrogenase (LDH) were elevated in the cartap-treated groups at or greater than 100 mu M. The isoenzymatic profiles showed that the elevations were mainly due to CK-3, LDH-3, and LDH-4, Following the addition of 0.5-2.5 mM EGTA, a Ca2+ chelator, or 30-100 mu M verapamil, an L-type Ca2+ channel blocker, the cartap-induced reduction in MTT metabolic rate of C2C12 cells was significantly restored in a dose-dependent manner in both EGTA and verapamil-treated cells. Furthermore, EGTA could significantly reduce the cartap-induced elevation in the levels of total extracellular CK and LDH activities. Additionally, cartap significantly increased the level of endogenous reactive oxygen species (ROS) in C2C12 cells in a dose- and time-dependent manner. The cartap-induced ROS generation could be significantly inhibited by antioxidants, including Vitamins C and E, catalase, and superoxide dismutase, with catalase the most effective. EGTA could significantly inhibit cartap-induced ROS generation in a dose-dependent manner. The results suggested that cartap could induce ROS generation in C2C12 cells via a Ca2+-dependant mechanism resulting in subsequent cytotoxicity, at least partially, to C2C12 cells. It is speculated that both Ca2+ and Ca2+-induced ROS may also play the central role on the myogenic contracture and myofiber injury of the diaphragm leading to respiratory failure and subsequent death in rabbits exposed ocularly to cartap. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
Relation: Toxicology
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 廖俊旺

loading Web of Knowledge data....

Files in This Item:

File SizeFormat
index.html0KbHTML337View/Open


 


學術資源

著作權聲明

本網站為收錄中興大學學術著作及學術產出,已積極向著作權人取得全文授權,並盡力防止侵害著作權人之權益。如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員,將盡速為您處理。

本網站之數位內容為國立中興大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用。

聯絡網站維護人員:wyhuang@nchu.edu.tw,04-22840290 # 412。

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU/NCHU Library IR team Copyright ©   - Feedback