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National Chung Hsing University Institutional Repository - NCHUIR > 生命科學院 > 基因體暨生物資訊學研究所 > 依資料類型分類 > 期刊論文 >  The binding of the Co(II) complex of dimeric chromomycin A3 to GC sites with flanking G:G mismatches

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/145712

標題: The binding of the Co(II) complex of dimeric chromomycin A3 to GC sites with flanking G:G mismatches
作者: Chen, Yu-Wen;Hou, Ming-Hon
Contributors: Wei Chun Wang
關鍵字: Groove-binding drugs;Dimeric chromomycin A3;Cobalt;CGG triplet repeats;DNA expansion
日期: 2013-11
Issue Date: 2013-07-02 10:20:20 (UTC+8)
摘要: Some neurological diseases are correlated with expansion of (CXG)n trinucleotide repeats, which contain many
contiguous GpC flanked bymismatched X/X base pair. This study focused on the binding of the Co(II) complex of
dimeric chromomycin A3(Chro), CoII(Chro)2, to DNA with CXG trinucleotide repeats. The present study showed
that GC siteswith flanking G:Gmismatches provide an excellent binding site for CoII(Chro)2 as shown by surface
plasmon resonance and fluorescence analysis, compared to GC sites with flanking A:A, T:T, or C:Cmismatches. In
addition, we measured the ability of CoII(Chro)2 to act on the hairpin DNA of (CGG)16. We observed that
CoII(Chro)2 could stabilize and trap the cruciform conformation of (CGG)16. Furthermore, two CoII(Chro)2
molecules may bind at the two GpC sites separated by at least one GC site in the hairpin structure of (CGG)16.
In a synthetic self-priming DNA model, 5′-(CGG)16(CCG)6-3′, CoII(Chro)2 can interfere with the expansion process
of CGG triplet repeats, as shown by a gel electrophoretic expansion assay. Here, we first report the acting
of CoII(Chro)2, the groove-binding drug, to trinucleotide repeats. Our results provide the possible biological consequence
of CoII(Chro)2 bound to CGG triplet repeat sequences.
Relation: Journal of Inorganic Biochemistry, Volume 121, page(s) 28–36.
Appears in Collections:[依資料類型分類] 期刊論文

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