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National Chung Hsing University Institutional Repository - NCHUIR > 生命科學院 > 基因體暨生物資訊學研究所 > 依資料類型分類 > 期刊論文 >  2,4,5-TMBA, a Natural Inhibitor of Cyclooxygenase-2, Suppresses Adipogenesis and Promotes Lipolysis in 3T3-L1 Adipocytes

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/145717

標題: 2,4,5-TMBA, a Natural Inhibitor of Cyclooxygenase-2, Suppresses Adipogenesis and Promotes Lipolysis in 3T3-L1 Adipocytes
作者: Wu, Man-Ru;Hou, Ming-Hon;Lin, Ya-Lin;Kuo, Chia-Feng
Contributors: Wei Chun Wang
關鍵字: 2,4,5-TMBA;3T3-L1 adipocyte;adipogenesis;lipolysis
日期: 2012-07
Issue Date: 2013-07-02 10:20:22 (UTC+8)
摘要: Obesity is a global health problem. Because of the high costs and side effects of obesity-treatment drugs, the
potential of natural products as alternatives for treating obesity is under exploration. 2,4,5-Trimethoxybenzaldehyde (2,4,5-
TMBA) present in plant roots, seeds, and leaves was reported to be a significant inhibitor of cyclooxygenase-2 (COX-2) activity
at the concentration of 100 μg/mL. Because COX-2 is associated with differentiation of preadipocytes, the murine 3T3-L1 cells
were cultured with 100 μg/mL of 2,4,5-TMBA during differentiation and after the cells were fully differentiated to study the
effect of 2,4,5-TMBA on adipogenesis and lipolysis. Oil Red O staining and triglyceride assay revealed that 2,4,5-TMBA inhibited
the formation of lipid droplets during differentiation; moreover, 2,4,5-TMBA down-regulated the protein levels of adipogenic
signaling molecules and transcription factors MAP kinase kinase (MEK), extracellular signal-regulated kinase (ERK), CCAAT/
enhancer binding protein (C/EBP)α, β, and δ, peroxisome proliferator-activated receptor (PPAR)γ, adipocyte determination and
differentiation-dependent factor 1 (ADD1), and the rate-limiting enzyme for lipid synthesis acetyl-CoA carboxylase (ACC). In
fully differentiated adipocytes, treatment with 2,4,5-TMBA for 72 h significantly decreased lipid accumulation by increasing the
hydrolysis of triglyceride through suppression of perilipin A (lipid droplet coating protein) and up-regulation of hormonesensitive
lipase (HSL). The results of this in vitro study will pioneer future in vivo studies on antiobesity effects of 2,4,5-TMBA
and selective COX-2 inhibitors.
Relation: J. Agric. Food Chem., Volume 60, page(s) 7262−7269.
Appears in Collections:[依資料類型分類] 期刊論文

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