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National Chung Hsing University Institutional Repository - NCHUIR > 學術研究中心 > 生物科技發展中心 > 依資料類型分類 > 期刊論文 >  High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/145832

標題: High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435
作者: Kuo, Chia-Hung;Chen, Hsin-Hung;Chen, Jiann-Hwa;Liu, Yung-Chuan;Shieh, Chwen-Jen
Contributors: Wei Chun Wang
關鍵字: optimization;wax esters;lipase;esterification;Rhizomucor miehei;Candida antarctica
日期: 2012-09
Issue Date: 2013-07-02 10:46:28 (UTC+8)
摘要: Wax esters are long-chain esters that have been widely applied in premium
lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic
preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two
commercial immobilized lipases, i.e., Lipozyme® RMIM (Rhizomucor miehei) and
Novozym® 435 (Candida antarctica). Response surface methodology (RSM) and
5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the
effects of reaction time (1–5 h), reaction temperature (45–65 °C), substrate molar ratio
(1–3:1), and enzyme amount (10%–50%) on the yield of cetyl octanoate. Using RSM to
optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme® RMIM
and Novozym® 435, respectively. The optimum conditions for synthesis of cetyl octanoate
by both lipases are established and compared. Novozym® 435 proves to be a more efficient
biocatalyst than Lipozyme® RMIM.
Relation: Int. J. Mol. Sci., Volume 13, Page(s) 11694-11704.
Appears in Collections:[依資料類型分類] 期刊論文
[依教師分類] 陳建華

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