English  |  正體中文  |  简体中文  |  Items with full text/Total items : 43312/67235
Visitors : 2149984      Online Users : 4
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU/NCHU Library IR team.
National Chung Hsing University Institutional Repository - NCHUIR > 學術研究中心 > 生物科技發展中心 > 依資料類型分類 > 期刊論文 >  PHO2-Dependent Degradation of PHO1 Modulates Phosphate Homeostasis in Arabidopsis

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/145836

標題: PHO2-Dependent Degradation of PHO1 Modulates Phosphate Homeostasis in Arabidopsis
作者: Liu, Tzu-Yin;Huang, Teng-Kuei;Tseng, Ching-Ying;Lai, Ya-Shiuan;Lin, Shu-I;Lin, Wei-Yi;Chen, June-Wei;Chiou, Tzyy-Jen
Contributors: Wei Chun Wang
日期: 2012-05
Issue Date: 2013-07-02 10:46:30 (UTC+8)
摘要: The Arabidopsis thaliana pho2 mutant, which is defective in a ubiquitin-conjugating E2 enzyme, displays inorganic phosphate
(Pi) toxicity as a result of enhanced uptake and root-to-shoot translocation of Pi. To elucidate downstream components of the
PHO2-dependent regulatory pathway, we identified two pho2 suppressors as carrying missense mutations in PHO1, which
has been implicated in Pi loading to the xylem. In support of the genetic interaction between PHO1 and PHO2, we found that
the protein level of PHO1 is increased in pho2, whereas such accumulation is ameliorated in both pho2 suppressors. Results
from cycloheximide and endosomal Cys protease inhibitor E-64d treatments further suggest that PHO1 degradation is PHO2
dependent and involves multivesicular body–mediated vacuolar proteolysis. Using the transient expression system of
tobacco (Nicotiana tabacum) leaves, we demonstrated that PHO1 and PHO2 are partially colocalized and physically interact
in the endomembranes, where the ubiquitin conjugase activity of PHO2 is required for PHO1 degradation. In addition, reduced
PHO1 expression caused by PHO1 mutations impede Pi uptake, indicating a functional association between xylem loading
and acquisition of Pi. Together, our findings uncover a pivotal molecular mechanism by which PHO2 modulates the
degradation of PHO1 in the endomembranes to maintain Pi homeostasis in plants.
Relation: The Plant Cell, Volume 24, Page(s) 2168–2183.
Appears in Collections:[依資料類型分類] 期刊論文

loading Web of Knowledge data....

Files in This Item:

File SizeFormat






聯絡網站維護人員:wyhuang@nchu.edu.tw,04-22840290 # 412。

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU/NCHU Library IR team Copyright ©   - Feedback