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National Chung Hsing University Institutional Repository - NCHUIR > 學術研究中心 > 生物科技發展中心 > 依資料類型分類 > 期刊論文 >  Rice LGD1 containing RNA binding activity affects growth and development through alternative promoters

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/145837

標題: Rice LGD1 containing RNA binding activity affects growth and development through alternative promoters
作者: Thangasamy, Saminathan;Chen, Pei-Wei;Lai, Ming-Hsing;Chen, Jychian;Jauh, Guang-Yuh
Contributors: Wei Chun Wang
關鍵字: Oryza sativa;growth and development;LGD1;multiple transcripts;vWA domain;RNA binding
日期: 2012
Issue Date: 2013-07-02 10:46:30 (UTC+8)
摘要: Tiller initiation and panicle development are important agronomical traits for grain production in Oryza
sativa L. (rice), but their regulatory mechanisms are not yet fully understood. In this study, T-DNA mutant and
RNAi transgenic approaches were used to functionally characterize a unique rice gene, LAGGING GROWTH
AND DEVELOPMENT 1 (LGD1). The lgd1 mutant showed slow growth, reduced tiller number and plant height,
altered panicle architecture and reduced grain yield. The fewer unelongated internodes and cells in lgd1 led to
respective reductions in tiller number and to semi-dwarfism. Several independent LGD1-RNAi lines exhibited
defective phenotypes similar to those observed in lgd1. Interestingly, LGD1 encodes multiple transcripts with
different transcription start sites (TSSs), which were validated by RNA ligase-mediated rapid amplification of 5�
and 3� cDNA ends (RLM-RACE). Additionally, GUS assays and a luciferase promoter assay confirmed the
promoter activities of LGD1.1 and LGD1.5. LGD1 encoding a von Willebrand factor type A (vWA) domain
containing protein is a single gene in rice that is seemingly specific to grasses. GFP-tagged LGD1 isoforms were
predominantly detected in the nucleus, and weakly in the cytoplasm. In vitro northwestern analysis showed
the RNA-binding activity of the recombinant C-terminal LGD1 protein. Our results demonstrated that LGD1
pleiotropically regulated rice vegetative growth and development through both the distinct spatiotemporal
expression patterns of its multiple transcripts and RNA binding activity. Hence, the study of LGD1 will
strengthen our understanding of the molecular basis of the multiple transcripts, and their corresponding
polypeptides with RNA binding activity, that regulate pleiotropic effects in rice.
Relation: Plant Journal, Volume 71, Page(s) 288–302.
Appears in Collections:[依資料類型分類] 期刊論文

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