English  |  正體中文  |  简体中文  |  Items with full text/Total items : 43312/67235
Visitors : 2027961      Online Users : 2
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU/NCHU Library IR team.
National Chung Hsing University Institutional Repository - NCHUIR > 學術研究中心 > 生物科技發展中心 > 依資料類型分類 > 期刊論文 >  Role of the Putative Osmosensor Arabidopsis Histidine Kinase1 in Dehydration Avoidance and Low-Water- Potential Response

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/145841

標題: Role of the Putative Osmosensor Arabidopsis Histidine Kinase1 in Dehydration Avoidance and Low-Water- Potential Response
作者: Kumar, M.Nagaraj;Jane, Wann-Neng;Paul, E.Verslues
Contributors: Wei Chun Wang
日期: 2012-11
Issue Date: 2013-07-02 10:46:32 (UTC+8)
摘要: The molecular basis of plant osmosensing remains unknown. Arabidopsis (Arabidopsis thaliana) Histidine Kinase1 (AHK1) can
complement the osmosensitivity of yeast (Saccharomyces cerevisiae) mutants lacking Synthetic Lethal of N-end rule1 and SH3-
containing Osmosensor osmosensors and has been proposed to act as a plant osmosensor. We found that ahk1 mutants in either
the Arabidopsis Nossen-0 or Columbia-0 background had increased stomatal density and stomatal index consistent with greater
transpirational water loss. However, the growth of ahk1 mutants was not more sensitive to controlled moderate low water
potential (cw) or to salt stress. Also, ahk1 mutants had increased, rather than reduced, solute accumulation across a range of low
cw severities. ahk1 mutants had reduced low cw induction of D1-Pyrroline-5-Carboxylate Synthetase1 (P5CS1) and 9-cis-
Epoxycarotenoid Dioxygenase3, which encode rate-limiting enzymes in proline and abscisic acid (ABA) synthesis, respectively.
However, neither Pro nor ABA accumulation was reduced in ahk1 mutants at low cw. P5CS1 protein level was not reduced in
ahk1 mutants. This indicated that proline accumulation was regulated in part by posttranscriptional control of P5CS1 that was
not affected by AHK1. Expression of AHK1 itself was reduced by low cw, in contrast to previous reports. These results define a
role of AHK1 in controlling stomatal density and the transcription of stress-responsive genes. These phenotypes may be
mediated in part by reduced ABA sensitivity. More rapid transpiration and water depletion can also explain the previously
reported sensitivity of ahk1 to uncontrolled soil drying. The unimpaired growth, ABA, proline, and solute accumulation of ahk1
mutants at low cw suggest that AHK1 may not be the main plant osmosensor required for low cw tolerance.
Relation: Plant Physiology, Volume 161, Page(s) 1-12.
Appears in Collections:[依資料類型分類] 期刊論文

loading Web of Knowledge data....

Files in This Item:

File SizeFormat
index.html0KbHTML165View/Open


 


學術資源

著作權聲明

本網站為收錄中興大學學術著作及學術產出,已積極向著作權人取得全文授權,並盡力防止侵害著作權人之權益。如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員,將盡速為您處理。

本網站之數位內容為國立中興大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用。

聯絡網站維護人員:wyhuang@nchu.edu.tw,04-22840290 # 412。

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU/NCHU Library IR team Copyright ©   - Feedback