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標題: 臺灣款冬及八角蓮藥用植物增殖系統之建立
Establishment of multiplication protocols on medicinal plants Petasites formosanus Kitam. and Dysosma pleiantha (Hance) Woodson
作者: 張慧如
Chang, Hui-Ju
Contributors: 廖松淵
生命科學院碩士在職專班
關鍵字: 臺灣款冬;八角蓮
Petasites formosanus Kitam.;Dysosma pleiantha (Hance) Woodson
日期: 2013
Issue Date: 2013-11-07 13:14:07 (UTC+8)
Publisher: 生命科學院碩士在職專班
摘要: 傳統的中醫醫學用藥是以植物性藥物為主,歷經幾千年的傳承不衰,而醫學發展至今,也逐漸重視中西醫的合併療法,藥用植物也隨之越受矚目。本實驗以臺灣原生種臺灣款冬植株及八角蓮為材料。臺灣款冬植株經由無菌培植體、芽體誘導、發根試驗及健化移植,建立臺灣款冬增殖系統。芽體誘導試驗以生長調節劑 BA比NAA為10比1的比例,濃度分別為BA 0.2 mg·L-1加NAA 0.02 mg·L-1、BA 0.4 mg·L-1加NAA 0.04 mg·L-1、BA 0.6 mg·L-1加NAA 0.06 mg·L-1及BA 0.8 mg·L-1加NAA 0.08 mg·L-1為試驗組;未添加任何生長調節劑的MS培養基作為對照組,測試結果以臺灣款冬培養於 MS培養機添加 BA 0.2 mg·L-1和NAA 0.02 mg·L-1,增殖芽數最多,但添加生長調節劑的植株易產生變異,故以MS為最佳;發根試驗以MS添加IBA 0.2 mg·L-1、MS添加IBA 0.5 mg·L-1、1/2 MS、1/2 MS添加IBA 0.2 mg·L-1、1/2 MS添加IBA 0.5 mg·L-1為試驗組;未添加任何生長調節劑的MS培養基作為對照組,試驗結果在第二週發根率達100%,其中以1/2 MS添加IBA 0.5 mg·L-1之發根數最多。八角蓮以不同鹽類組成之培養基及各種生長調節劑添加,僅產生癒傷組織,其中以MS培養基添加 BA 2.0 mg·L-1及TDZ 0.2 mg·L-1誘導之癒傷組織可持續增生且不會褐化死亡,有待後續研究。
Botanical drugs are mainly applied by the traditional Chinese medical science for thousands of years, it is passed on and never-ending. The medical science pays much attention to the combined therapy with Chinese and Western medicine gradually since developed, and focuses on medical plants. The aims of this study were to establish in vitro multiplication protocol of Taiwan native species, Petasites formosanus Kitam. and Dysosma pleiantha (Hance) Woodson. The processes include establishing aseptic explants,inducing shoots,rooting and transplanting. In the inducing shoots test, the test of Petasites formosanus Kitam. were BA 0.2 mg·L-1+NAA 0.02 mg·L-1,BA 0.4 mg·L-1+NAA 0.04 mg·L-1,BA 0.6 mg·L-1+NAA 0.06 mg·L-1,BA 0.8 mg·L-1+NAA 0.08 mg·L-1, with BA-NAA ratio 10:1 and the Murashige and Skoog (MS) medium was the control group.In vitro micropropagation of Petasites formosanus Kitam. was established to get maximum shoots multiplication rate by cultivating axillary buds on MS supplemented with BA 0.2 mg·L-1+ NAA 0.02 mg·L-1,but added plant growth regulators suppressing growth of explants and cause variation. In the rooting test, the test of Petasites formosanus Kitam. were MS, MS with 0.2 mg·L-1 IBA, MS with 0.5 mg·L-1 IBA ,1/2 MS, 1/2 MS with 0.2 mg·L-1 IBA, 1/2MS with 0.5 mg·L-1 IBA. Rooting rate achieved 100% by cultivating on MS plant medium for 2 weeks. 1/2MS with 0.5 mg·L-1 IBA has the most roots. Dysosma pleiantha (Hance) Woodson plant got the callus after being cultivated with different media and growth regulators, in which MS media with BA 2.0 mg·L-1+TDZ 0.2 mg·L-1induction of callus can continuously proliferate without browning and having died back, later generations to study.
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