English  |  正體中文  |  简体中文  |  Items with full text/Total items : 43312/67235
Visitors : 2106602      Online Users : 3
RC Version 5.0 © Powered By DSPACE, MIT. Enhanced by NTU/NCHU Library IR team.
National Chung Hsing University Institutional Repository - NCHUIR > 工學院 > 生醫工程研究所 > 依資料類型分類 > 碩博士論文 >  BODIPY衍生物之合成、光學性質探討與光動力治療之應用

Please use this identifier to cite or link to this item: http://nchuir.lib.nchu.edu.tw/handle/309270000/152682

標題: BODIPY衍生物之合成、光學性質探討與光動力治療之應用
Synthesis and Optical Investigation of BODIPY-derivative for Selective Photodynamic Therapy
作者: 賴彥池
Lai, Yen-Chih
Contributors: 張健忠
生醫工程研究所
關鍵字: 光動力治療;光穩定性
PDT;BODIPY;Photostability
日期: 2013
Issue Date: 2013-11-18 11:11:18 (UTC+8)
Publisher: 生醫工程研究所
摘要: 在此論文中,我們成功合成出一系列以BODIPY 為主體並具有光穩定和高光
學效率特性之光敏感物質,而這些光敏感物質具有偵測酸濃度、在光源照射下同
時產生單重態氧與超氧化物之能力。另外,藉由調控合成步驟搭配使用較便宜之
起始物,我們可以很輕易的合成出不同取代位置之BODIPY 衍生物。此外,在
生物實驗中,藉由觀察化合物在細胞中的攝取量、所待胞器位置和光毒性,我們
可以發現到化合物5b 和8a 在光動力治療中對於癌細胞的毒殺能力遠大於正常細
胞,且沒有觀察到明顯之暗毒性,因此我們認為化合物5b 和8a 在選擇性光動力
治療上是很有潛力的,而在文獻中,BODIPY 分子具有此特性是很罕見的。另外,
跟市售之光敏感物質做比較,可發現所合成出來之化合物與methylene blue 具有
相似之光毒性效果,而遠大於Foscan。
BODIPY 分子具有許多卓越的光學特性。在本研究中,透過觀察溶液的顏色變
化與螢光增強現象,具有可酸化官能基的化合物可被應用於在水溶液和有機溶劑
中偵測酸濃度之變化。此外,我們成功的將BODIPY 衍生物應用於癌症標靶上,
並證明化合物5b 和8a 在光動力治療中對腫瘤專一性方面是具有潛力的光敏感物
質。因此,在此研究中主要有以下特色,第一:我們提出便宜又方便的合成步驟,
可廣泛的應用於多元的BODIPY 衍生物合成。第二:藉由觀察ROS 的產生、細
胞對化合物之攝取量、化合物在細胞中所待的位置和光毒性表現,可以發現化合
物5b 和8a 具有以下特性:(1)在光動力治療中對癌細胞具有專一性,(2)相對於
methylene blue 具有較佳的光穩定性,(3)光毒性效果與methylene blue 幾乎一致
而遠高於Foscan,(4)超氧化物的產生以提供額外之光毒性。
We introduce a new class of photostable, efficient photosensitizer base on BODIPY
derivatives which can be used to detect acid concentration, generate singlet oxygen
and super oxide simultaneously under irradiation. Appropriate regulation of the
synthesis procedure coupled with cheaper materials, we can facilitate controlling the
generation of specifically substituted position of BODIPY derivatives. Biologically
evaluate the uptake, localization and phototoxicity in cell lines, we conclude that
compounds 5b and 8a are potential selective PDT candidates due to their
photodynamic therapy are more efficient in cancer cell more than normal cell without
significant dark toxicity. Based on our knowledge, this is very rare for PDT
application of BODIPY derivatives. Furthermore, direct comparison of the
photodamage efficacy, our compound is better than Foscan and nearly equal to
methylene blue.
BODIPY offered many superior optical properties. In this study, by observing the
color change and fluorescence enhancement of solution , compounds with proton
accepted function groups can be used to detect acid concentration in organic solvent
and aqueous solution. Besides we have successfully applied BODIPY derivatives for
cancer targeting and demonstrated that compound 5b and 8a may be potent
tumor-specific photosensitizers for PDT. First, we presented that cheap and
convenient synthesis procedure which can be widely applied for the preparation of
diversified BODIPY derivatives. Second, biological evolution of the ROS generation
and cellular uptake, localization and phototoxicity, therefore, the PDT characteristics
of the compound 5b and 8a include (1) target selectivity for the PDT of cancer cells;
(2) high photostability than methylene blue; (3) photodamage efficiency is much
better than Foscan and nearly equal to MB; (4) supported extra phototoxicity with
super oxide generation.
Appears in Collections:[依資料類型分類] 碩博士論文

Files in This Item:

File SizeFormat
index.html0KbHTML351View/Open


 


學術資源

著作權聲明

本網站為收錄中興大學學術著作及學術產出,已積極向著作權人取得全文授權,並盡力防止侵害著作權人之權益。如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員,將盡速為您處理。

本網站之數位內容為國立中興大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用。

聯絡網站維護人員:wyhuang@nchu.edu.tw,04-22840290 # 412。

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU/NCHU Library IR team Copyright ©   - Feedback